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CY5-4040A Fluorescence Filter Set – Fluorophores

Table of fluorophores that may be used with the BrightLine® Cy5™ filter set:   The set is optimized for use with the fluorophores highlighted in bold. Note that results may vary depending upon instrumentation and conditions.
Fluorophore Peak Excitation
Wavelength* (nm)
Peak Emission
Wavelength* (nm)
 Alexa Fluor 647™ 647 666
 Alexa Fluor 660™ 663 690
 APC (Allophycocyanin) 651 661
 BODIPY 650/665 Dye 650 665
 BODIPY 650/665-X 647 665
 Cy5™ 649 666
 DiD (DiIC18(5)) - Lipophilic Tracer 644 665
 DY-635-NHS 634 664
 Indodicarbocyanine (DiD) 644 665
 Quasar 670 644 670
 SpectrumFRed (Far Red) 655 675
 SYTO Red Fluorescent Nucleic Acid Stains 60, 62, 63 655 675
 Thiadicarbocyanine (DiSC3) 651 674
 TOTO-3, TO-PRO-3 642 661
 Ultralite 656 678
* These are typical wavelengths to be used for guidance purposes only. Actual peak wavelengths may vary for different applications.
 
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