This
filter set is ideal for use with Cy3™ and a variety
of other orange fluorophores (including DsRed fluorescent
protein). It is optimized to provide the highest brightness,
while maintaining a very high signal-to-noise ratio.
Filter
Average
Transmission
Center
Wavelength
Bandwidth
*
Exciter
>
93%
531
nm
40
nm
Emitter
>
93%
593
nm
40
nm
Average
Reflection
Reflection
Band
Transmission
Band
Dichroic
>
98%
499
- 555 nm
569
- 730 nm
* Bandwidth
is the minimum width over which the average transmission
exceeds the specified passband transmission; the nominal
full-width-at-half-maximum (FWHM) is approximately the
Bandwidth + 1% of the Center Wavelength.
Are
you co-labeling with YFP, GFP, or FITC? In order to substantially
eliminate green-fluorophore bleedthrough we recommend using our
TRITC-A filter set. For mercury
(e.g., HBO) lamp users labeling with Cy3 the single-fluorophore
signal-to-noise ratio will be slightly higher, and the brightness
slightly lower, with the TRITC-A filter set. Xenon lamp users
of the TRITC-A filter set who are labeling with Cy3 will enjoy
a significantly enhanced signal-to-noise ratio and reduced photobleaching
in exchange for brightness.
Exciter
Dichroic
Emitter
