| |
 |
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|
 TRITC
Basic Filter Set |
|
|
|
| Optimized
for these fluorophores |
| TRITC,
dTomato, Alexa Fluor 546, Rhodamine, DiI |
|
|
| Actual
measured data from typical filters |
|
|
This
filter set is ideal for use with TRITC and other similar
fluorophores. When viewing TRITC visually, it has the "reddish"
appearance with which many experienced microscopists are
familiar. This hard-coated filter set is the best value
on the market, delivering high brightness and contrast. |
|
| Filter |
Average
Transmission |
Center
Wavelength |
Bandwidth
* |
| Exciter |
>
90% |
542
nm |
20
nm |
| Emitter |
>
90% |
620
nm |
52
nm |
| |
Average
Reflection |
Reflection
Band |
Transmission
Band |
| Dichroic |
>
90% |
525
- 556 nm |
580
- 650 nm |
|
| * Bandwidth
is the minimum width over which the average transmission
exceeds the specified passband transmission; the nominal
full-width-at-half-maximum (FWHM) is approximately the
Bandwidth + 1% of the Center Wavelength. |
|
|
| Compare BrightLine
Basic filters to soft-coated filter sets and our highest-performance
BrightLine filter sets. |
|
Note: Historical
limitations in filter technology led to the widespread
use of TRITC emission filters that are overly broad
and red-shifted,
causing the TRITC fluorescence to appear red. This BrightLine
Basic filter set delivers that familiar "reddish"
appearance. In contrast, Semrock’s highest-performance
BrightLine TRITC-A filter
set is optimized for the best signal and noise performance,
and since the TRITC emission band peaks in the orange,
the
fluorescence correctly appears orange – not red. |
|
|
|
Exciter
Dichroic
Emitter
