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BrightLine® Multiphoton

Fluorescence Filters


These ultrahigh-performance BrightLine multiphoton fluorescence filters serve a full range of applications with just two wavelength ranges. The filters conveniently accommodate the wide range of fluorescent dyes that are the essential tools of the modern researcher. The transmission bands of these state-of-the-art emitters are so wide that these filters appear clear at normal incidence, in contrast to the yellowish color of older restricted-range multiphoton filters. And the reflection bands of these stunning long-wave-pass dichroics are so wide that they look like highly reflective mirrors!

As with all BrightLine filters, Semrock’s proprietary Ion Beam Sputtering process means that these multiphoton filters offer you amazing transmission, steep edges, and exceptional blocking. In fact, BrightLine multiphoton filter technology is so unique that it is patent pending. State-of-the-art performance, "no burn-out" reliability, reduced component count – you have invested so much into multiphoton microscopy, why not use the best filters available?

For more information, read our cover story Optical Filters for Multiphoton Microscopy from the November 2006 issue of BIOPHOTONICS (691 KB)

Tutorial: Multiphoton fluorescence microscopy

The difference is clear!

Need an ultrabroadband mirror for your multiphoton system? Try our MaxMirror

 Part Number
Filter Type
Fluorescence/Signal Band
Laser/Excitation Band
 Near-UV & Visible Filters Emitter filter has exceptional guaranteed OD > 8 blocking for detecting the lowest fluorophore
 concentrations, with transmission even down to near-UV wavelengths.
 FF01-680/SP-25
 Emission Filter
350 – 650 nm
680 – 1080 nm
 FF665-Di02-25x36
 Dichroic Long-wave-pass (laser-transmitting)
350 – 650 nm
680 – 1080 nm
 FF670-SDi01-25x36
 Dichroic Short-wave-pass (laser-reflecting)
360 – 650 nm
680 – 1080 nm
 Full Visible Filters Excellent detection of fluorescence throughout the full visible wavelength range  including "red" fluorophores
 like Cy5™
with solid OD > 6 blocking over the entire laser band.
 FF01-750/SP-25
 Emission Filter
380 – 720 nm
750 – 1100 nm
 FF735-Di01-25x36
 Dichroic Long-wave-pass (laser-transmitting)
380 – 720 nm
750 – 1100 nm
 FF720-SDi01-25x36
 Dichroic Short-wave-pass (laser-reflecting)
 ... Ideal for SHG imaging
370 – 690 nm
750 – 875 nm
Laser-transmitting configuration
Uses a long-wave-pass dichroic beamsplitter
Laser-reflecting configuration
Uses a short-wave-pass dichroic beamsplitter
(FF665-Di02 or FF735-Di01) (FF670-SDi01 or FF720-SDi01)

Filter spectra – Near-UV & Visible Filters

Laser Blocking Emission Filter – FF01-680/SP-25
Compatible Dichroic Beamsplitters:  FF665-Di02, FF670-SDi01
 Fluorescence Transmission Range
 T (average) > 90%
350 – 650 nm
 Laser Blocking Range
OD > 8:  680 – 1040 nm
OD > 6:  1040 – 1080 nm
 Size
 25.0 mm x 3.5 mm
 Price
$995
 

Dichroic Long Wave Pass Beamsplitter – FF665-Di02-25x36
Compatible Emission Filter:  FF01-680/SP
 Transmitted Laser Wavelengths
 T (average) > 90%
 680 – 1600 nm
 Reflection Bands
 R (average) > 98%
 350 – 650 nm
 Size (Length x Width x Thickness)
 25.2 mm x 35.6 mm x 1.1 mm
 Price
$495  Price reduced!
 

Dichroic Short Wave Pass Beamsplitter – FF670-SDi01-25x36
Compatible Emission Filter:  FF01-680/SP
 Transmitted Laser Wavelengths
 T (average) > 90%
 360 – 650 nm
 Reflection Bands
 R (average) > 98% (s-polarization)
 R (average) > 90% (p-polarization)

680 – 1080 nm
700 – 1010 nm
 Group Delay Dispersion
 Polarization Dependence
 Size (Length x Width x Thickness)
 25.2 mm x 35.6 mm x 1.1 mm
 Price
$495  Price reduced!
 
Filter spectra – Full Visible Filters

Laser Blocking Emission Filter – FF01-750/SP-25
Compatible Dichroic Beamsplitters:  FF720-SDi01, FF735-Di01
 Fluorescence Transmission Range
 T (average) > 90%
380 – -720 nm
 Laser Blocking Range
OD > 6:  750 – 1100 nm
 Size
 25.0 mm x 3.5 mm
 Price
$795
 

Excellent dispersion and polarization-preserving properties make this filter ideal for imaging Second Harmonic Generation (SHG)!
NEW! ... Dichroic Short Wave Pass Beamsplitter – FF720-SDi01-25x36
Compatible Emission Filter:  FF01-750/SP
 Transmitted Laser Wavelengths
 T > 85% (average polarization)
 T > 90% (s- & p-polarizations)

370 – 690 nm
400 – 410 nm
 Reflection Bands
 R (average) > 95% (average polarization)
 R > 99% (s- & p-polarizations)


750 – 875 nm
800 – 820 nm

 Group Delay Dispersion
 Polarization Dependence
 Size (Length x Width x Thickness)
25.2 mm x 35.6 mm x 1.1 mm
 Price
$495 
ASCII data

Dichroic Beamsplitter – FF735-Di01-25x36
Compatible Emission Filter:  FF01-750/SP
 Transmitted Laser Wavelengths
 T (average) > 90%
 750 – 1100 nm
 Reflection Bands
 R (average) > 98%
350 – 720 nm
 Size (Length x Width x Thickness)
25.2 mm x 35.6 mm x 1.1 mm
 Price
$495  Price reduced!
 
 

 

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