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BrightLine^® Multi–edge Dichroic Beamsplitter Catalog

The highest performance fluorescence filters available … and the choice of major OEM’s worldwide!

These polarization–insensitive dichroic beamsplitters for 45° angle–of–incidence exhibit steep edges with very high and flat reflection and transmission bands. More complete reflection and transmission mean less stray light for lower background and improved signal–to–noise ratio. These filters are optimized for fluorescence microscopes and instrumentation, and may also be used for a variety of other applications that require beam combining and separation based on wavelength. All dichroic filters on this page utilize Semrock's highly reliable hard–coating technology on ultralow–autofluorescence fused–silica substrates.

For more information on how to properly orient our dichroic beamsplitters, refer to orientation information.

We offer custom-sizing in one week.

Multiedge dichroic beamsplitters [also see our single–edge dichroic beamsplitters]

Edge Color

Filter

Nominal Edge
Wavelengths

Reflection Bands *

Transmission Bands *

Special Features

Size

Price

FF440/520-Di01-25x36

440 nm
520 nm

415 – 432 nm
493 – 511 nm

449 – 483 nm
530 – 569 nm

25.2 mm x 35.6 mm
x 1.1 mm

$275

FF462/523-Di01-25x36

462 nm
523 nm

430 – 453 nm
508 – 512 nm

471 – 489 nm
534 – 650 nm

25.2 mm x 35.6 mm
x 1.1 mm

$350

FF493/574-Di01-25x36

493 nm
574 nm

456 – 480 nm
541 – 565 nm

500 – 529 nm
584 – 679 nm

25.2 mm x 35.6 mm
x 1.1 mm

$275

FF495/605-Di01-25x36

495 nm
605 nm

454 – 485 nm
570 – 598 nm

505 – 550 nm
620 – 675 nm

25.2 mm x 35.6 mm
x 1.1 mm

$350

FF497/554-Di01-25x36

497 nm
554 nm

486 – 490 nm
542 – 544 nm

420 – 471 nm
505 – 525 nm
561 – 700 nm

Designed for blocking laser wavelengths 488 nm and 543 nm

25.2 mm x 35.6 mm
x 1.1 mm

$350

FF497/661-Di01-25x36

497 nm
661 nm

486 – 490 nm
646 – 648 nm

420 – 471 nm
505 – 626 nm
668 – 750 nm

Designed for blocking laser wavelengths 488 nm and 647 nm

25.2 mm x 35.6 mm
x 1.1 mm

$350

FF498/581-Di01-25x36

498 nm
581 nm

486 – 490 nm
567 – 569 nm

420 – 471 nm
505 – 549 nm
587 – 700 nm

Designed for blocking laser wavelengths 488 nm and 568 nm

25.2 mm x 35.6 mm
x 1.1 mm

$350

FF500/646-Di01-25x36

500 nm
646 nm

486 – 490 nm
632 – 634 nm

420 – 471 nm
505 – 613 nm
653 – 750 nm

Designed for reflecting laser wavelengths 488 nm and 633 nm

25.2 mm x 35.6 mm
x 1.1 mm

$350

FF502/670-Di01-
25x36x3.0

502 nm
670 nm

350 – 494 nm
641 – 660 nm

505 – 621 nm
677 – 800 nm

Designed for reflecting laser wavelengths 488 nm and 664 nm

25.2 mm x 35.6 mm
x 3.0 mm

$350

FF505/606-Di01-25x36

505 nm
606 nm

458 – 499 nm
570 – 600 nm

509 – 541 nm
612 – 647 nm

25.2 mm x 35.6 mm
x 1.1 mm

$275

FF545/650-Di01-25x36

545 nm
650 nm

532.0 nm
632.8 nm

554 – 613 nm
658 – 742 nm

Designed for dual–laser excitation (532 and 633 nm) of Cy3 and Cy5

25.2 mm x 35.6 mm
x 1.1 mm

$275

FF553/659-Di01-25x36

553 nm
659 nm

542 – 544 nm
646 – 648 nm

420 – 525 nm
561 – 626 nm
668 – 750 nm

Designed for reflecting laser wavelengths 543 nm and 647 nm

25.2 mm x 35.6 mm
x 1.1 mm

$350

FF555/646-Di01-25x36

555 nm
646 nm

542 – 544 nm
632 – 634 nm

420 – 525 nm
561 – 613 nm
653 – 750 nm

Designed for reflecting laser wavelengths 543 nm and 633 nm

25.2 mm x 35.6 mm
x 1.1 mm

$350

FF560/659-Di01-25x36

560 nm
659 nm

514 – 553 nm
617 – 652 nm

564 – 591 nm
665 – 718 nm

25.2 mm x 35.6 mm
x 1.1 mm

$275

FF576/661-Di01-25x36

576 nm
661 nm

567 – 569 nm
646 – 648 nm

420 – 549 nm
587 – 626 nm
668 – 750 nm

Designed for reflecting laser wavelengths 568 nm and 647 nm

25.2 mm x 35.6 mm
x 1.1 mm

$350

FF579/644-Di01-25x36

579 nm
644 nm

567 – 569 nm
632 – 634 nm

420 – 549 nm
587 – 613 nm
653 – 750 nm

Designed for reflecting laser wavelengths 568 nm and 633 nm

25.2 mm x 35.6 mm
x 1.1 mm

$350

FF395/495/610-Di01-25x36

395 nm
495 nm
610 nm

354 – 385 nm
465 – 483 nm
570 – 596 nm

403 – 446 nm
502 – 552 nm
620 – 750 nm

25.2 mm x 35.6 mm
x 1.1 mm

$350

FF436/514/604-Di01-25x36

436 nm
514 nm
604 nm

394 – 414 nm
484 – 504 nm
566 – 586 nm

446 – 468 nm
520 – 540 nm
614 – 642 nm

Designed for simultaneous excitation and detection of DAPI, FITC and Texas Red (or other blue, green and red dye combinations)

25.2 mm x 35.6 mm
x 1.1 mm

$350

FF444/520/590-Di01-25x36

444 nm
520 nm
590 nm

327 – 437 nm
494 – 512 nm
562 – 578 nm

450 – 480 nm
527 – 547 nm
598 – 648 nm

25.2 mm x 35.6 mm
x 1.1 mm

$350

FF444/521/608-Di01-25x36

444 nm
521 nm
608 nm

420 – 430 nm
496 – 510 nm
579 – 596 nm

451 – 480 nm
530 – 561 nm
618 – 664 nm

25.2 mm x 35.6 mm
x 1.1 mm

$350

FF462/522/607-Di01-25x36

462 nm
522 nm
607 nm

403 – 453 nm
507 – 512 nm
580 – 595 nm

471 – 489 nm
532 – 560 nm
619 – 750 nm

25.2 x 35.6 x 1.1 mm

$350

FF494/540/650
-Di01-25x36x3.5

494 nm
540 nm
650 nm

488 nm
(s pol only)
532 nm
633 – 642 nm

500 – 519 nm
545 – 610 nm
655 – 700 nm

NBK7 substrate

25.2 x 35.6 x 3.5 mm

$350

FF410/504/582/669-
Di01-25x36

410 nm
504 nm
582 nm
669 nm

381 – 392 nm
475 – 495 nm
547 – 572 nm
643 – 656 nm

420 – 460 nm
510 – 531 nm
589 – 623 nm
677 – 722 nm

Designed for simultaneous excitation and detection of DAPI, FITC, TRITC or Texas Red, and Cy5 (or other blue, green, orange, and red dye combinations)

25.2 mm x 35.6 mm
x 1.1 mm

$425

FF416/500/582/657
-Di01-25x36x1.5

416 nm
500 nm
582 nm
657 nm

400 - 410 nm
486 - 491 nm
560 - 570 nm
633 - 647 nm

422 - 473 nm
506 - 545 nm
586 - 617 nm
666 - 750 nm

25 mm x 36 mm x 1.5 mm

$425

* Wavelength ranges over which average reflection and transmission are guaranteed to be above 95% and 90%, respectively.

Laser–transmitting dichroic beamsplitters*

These beamsplitters have high and narrow transmission bands centered on popular laser lines while reflecting fluorescence over the broad reflection bands. They are ideal for high–quality imaging with better than 0.75λ flatness over the full aperture.

Laser Lines

Filter

Transmitted Laser Wavelengths *

Reflection Bands *

Special Features

Size

Price

Di01-T488/532/638
-25x36x5.0

³ 90% 488 nm, 532 nm, (636 – 640 nm)

³ 90% (499 – 521 nm) (543 – 623 nm)
(653 – 755 nm)

25.2 mm x 35.6 mm
x 5.0 mm

$500

Di01-T488/568/638
-25x36x5.0

³ 90% 488 nm, 568 nm, (636 – 640 nm)

³ 90% (499 – 556 nm)
(580 – 622 nm)
( 652 – 755 nm)

25.2 mm x 35.6 mm
x 5.0 mm

$500

Di01-T488/532/568/638
-25x36x5.0

³ 90% 488 nm, 532 nm, 568 nm, (636 – 640 nm)

³ 90% (499 – 521 nm) (543 – 556 nm)
(580 – 622 nm)
(652 – 755 nm)

25.2 mm x 35.6 mm
x 5.0 mm

$575

Di01-T488/532/594/638
-25x36x5.0

³ 90% 488 nm, 532 nm, 594 nm, (636 – 640 nm)

³ 90% (499 – 521 nm)
(543 – 580 nm)
(608 – 623 nm)
(653 – 755 nm)

25.2 mm x 35.6 mm
x 5.0 mm

$575

* Wavelengths and ranges over which the transmission and average reflection are guaranteed to be above 90%.

Don't see a part number you've ordered previously? Semrock is constantly increasing and improving our product offerings. See the
Filter Evolution Table for a list of replacement part numbers.

For complete fluorescence filter sets, see our main BrightLine Filter Catalog.

In addition to our standard catalog filters, Semrock also tailors optical filters to meet or exceed even the most demanding requirements of our volume OEM customers. Please contact us to discuss your requirements.