Semrock

Fluorophore Cross-reference Table

This table lists fluorophores that may be used with single-band BrightLine® filter sets. Note that results may vary. To see the fluorophores for which individual BrightLine filter sets are optimized, refer to the list on the main page for each filter set.

Fluorophore	Peak Excitation Wavelength* (nm)	Peak Emission Wavelength* (nm)	Recommended BrightLine Filter Set
1,8-ANS	372	480	DAPI-1160A or DAPI-5060B
4-methylumbelliferone	360	440	DAPI-1160A or DAPI-5060B
5-carboxy-2,7-dichlorofluorescein	504	529	FITC-3540B
5-carboxynapthofluorescein (pH 10)	555	615	TXRED-4040B
5-FAM (5-carboxyfluorescein)	492	518	FITC-3540B
5-ROX (carboxy-X-rhodamine)	580	605	TXRED-4040B or TXRED-A-Basic
5-TAMRA (5-carboxytetramethylrhodamine, high pH > 8)	542	568	TRITC-A
6-Carboxyrhodamine 6G	518	543	YFP-2427A
6-FAM	492	515	FITC-3540B
7-AAD	546	647	TXRED-4040B
7-amino-4-methylcoumarin	351	430	DAPI-1160A or DAPI-5060B
7-aminoactinomycin D (7-AAD)	546	647	TXRED-4040B
7-hydroxy-4-methylcoumarin	360	449	DAPI-1160A or DAPI-5060B
ABQ	344	445	DAPI-1160A or DAPI-5060B
AcGFP1	475	505	GFP-3035B
Acid Fuchsin	540	630	TXRED-4040B
ACMA (9-amino-6-chloro-2-methoxyacridine)	421	473	CFP-2432A
Acridine	362	462	FITC-3540B
Acridine Orange	500	526	FITC-3540B
Acridine Orange + DNA	502	526	FITC-3540B
Acridine Yellow	470	550	FITC-3540B
Alexa Fluor 350™	346	442	DAPI-1160A or DAPI-5060B or BFP-A-Basic
Alexa Fluor 405™	402	421	DAPI-1160A or DAPI-5060B
Alexa Fluor 488™	499	520	FITC-3540B or FITC-A-Basic
Alexa Fluor 532™	532	553	TRITC-A
Alexa Fluor 546™	556	573	TRITC-A or TRITC-A-Basic
Alexa Fluor 555™	555	565	CY3-4040B
Alexa Fluor 568™	577	603	TXRED-4040B or TXRED-A-Basic
Alexa Fluor 594™	590	617	TXRED-4040B or TXRED-A-Basic
Alexa Fluor 647™	647	666	CY5-4040A
Alexa Fluor 660™	663	690	CY5-4040A
Alexa Fluor 680™	679	702	CY5.5-A
Alexa Fluor 750™	750	780	CY7-A
Alizarin Red	540	580	TRITC-A
AMCA (Aminomethylcoumarin)	345	425	DAPI-1160A or DAPI-5060B or BFP-A-Basic
AMCA-X	353	442	DAPI-1160A or DAPI-5060B or BFP-A-Basic
AmCyan	454	488	CFP-2432A
AmCyan1	458	489	CFP-2432A
Aminoactinomycin D	555	655	TXRED-4040B
Aminocoumarin	350	445	DAPI-1160A or DAPI-5060B
Anthrocyl stearate	370	446	DAPI-1160A or DAPI-5060B
APC (Allophycocyanin)	651	661	CY5-4040A
Aspirin	240	380	TRP-A
AsRed 2	578	592	TXRED-4040B
Astrazon Brilliant Red 4G	500	585	TRITC-A
Astrazon Orange R	470	540	FITC-3540B
Astrazon Yellow 7 GLL	450	480	CFP-2432A
Atabrine	436	490	CFP-2432A
ATTO-TAG™ CBQCA	465	560	FITC-3540B
Auramine	460	550	FITC-3540B
Aurophosphine	470	515	GFP-3035B
Beta lactamase	409	450	DAPI-1160A or DAPI-5060B
BFP (Blue Fluorescent Protein)	382	448	DAPI-1160A or DAPI-5060B or BFP-A-Basic
Biphenyl	270	385	TRP-A
Bisbenzimide (Hoechst)	360	461	DAPI-1160A or DAPI-5060B
bis-BTC	455	529	GFP-3035B
Blancophor FFG	390	470	DAPI-1160A or DAPI-5060B
Blancophor SV	370	435	DAPI-1160A or DAPI-5060B
BOBO-1, BO-PRO-1	462	481	CFP-2432A or CFP-A-Basic
BOBO™-3	570	602	CY3-4040B
BODIPY	505	513	FITC-3540B or FITC-A-Basic
BODIPY 492/515	490	515	FITC-3540B
BODIPY 505/515	502	510	FITC-3540B
BODIPY 542/563	543	563	TRITC-A
BODIPY 564/570	564	570	CY3-4040B
BODIPY 650/665 Dye	650	665	CY5-4040A
BODIPY 650/665-X	647	665	CY5-4040A
BODIPY FL-Br2	531	545	YFP-2427A
BODIPY TMR	542	574	TRITC-A
BODIPY TR	588	616	TXRED-4040B
BODIPY TR ATP	591	620	TXRED-4040B
BODIPY TR-X dye	589	617	TXRED-4040B
BODIPY TR-X SE	588	616	TXRED-4040B
BO-PRO™-3	544	570	TRITC-A
BTC	464	530	FITC-3540B
Calcein	494	517	FITC-3540B
Calcein Blue	373	440	DAPI-1160A or DAPI-5060B
Calcium Crimson™	588	611	TXRED-4040B
Calcium Green-1	506	531	YFP-2427A or YFP-A-Basic
Calcium Green-2 (including Ca2+)	506	536	YFP-2427A
Calcium Green-5N (including Ca2+)	506	532	YFP-2427A
Calcium Orange	549	575	CY3-4040B
Calcofluor White	350	440	DAPI-1160A or DAPI-5060B CFW-LP01 or CFW-BP01
CAL Fluor® Gold 540	522	544	YFP-2427A
CAL Fluor Orange 560	538	559	TRITC-A
CAL Fluor Red 590	569	591	Cy3-4040B
CAL Fluor Red 610	590	610	TxRed-4040B
Carboxy SNARF Indicators	548	587	CY3-4040B
Cascade Blue™	377	420	DAPI-1160A or DAPI-5060B
Catecholamine	410	470	CFP-2432A
CFDA	494	520	FITC-3540B
CFP (cyan GFP)	434	477	CFP-2432A or CFP-A-Basic
Chromomycin A	448	470	CFP-2432A
CI-NERF, CL-NERF	509	540	YFP-2427A
CMFDA	494	520	FITC-3540B
Coelenterazine F	437	473	CFP-2432A
Coumarin Phalloidin	387	470	DAPI-1160A or DAPI-5060B
CPM Methylcoumarin	384	469	DAPI-1160A or DAPI-5060B
Cerulean	434	475	CFP-2432A or CFP-A-Basic
CoralHue Kusabira Orange	548	559	CY3-4040B
Cy2™	489	506	GFP-3035B or GFP-A-Basic
Cy3™	552	570	CY3-4040B
Cy3.5™	580	591	TXRED-4040B or Cy3.5-A-Basic
Cy5™	649	666	CY5-4040A
Cy5.5™	676	705	CY5.5-A
Cy7™	743	767	CY7-A
Cyclic AMP Fluorosensor (FiCRhR)	500	517	FITC-3540B
CyQuant Cell Proliferation Assay	480	520	GFP-3035B
DAPI	345	455	DAPI-1160A or DAPI-5060B or BFP-A-Basic
DCFDA	504	529	FITC-3540B
DCFH (Dichlorodihydrofluorescein Diacetate)	505	535	YFP-2427A
DEAC	432	472	SpAqua-A
DHR (Dihydrorhodamine 123)	505	534	YFP-2427A
DiD (DiIC18(5)) - Lipophilic Tracer	644	665	CY5-4040A
DiI	551	565	TRITC-A or TRITC-A-Basic
DiI (DiIC18(3))	549	565	TRITC-A
DiO	475	500	GFP-3035B or GFP-A-Basic
DM-NERF	500	540	YFP-2427A
DsRed Monomer	556	586	CY3-4040B
DsRed2	563	582	CY3-4040B
DsRed-Express	557	579	CY3-4040B
DTAF	494	520	FITC-3540B
dTomato	554	581	TRITC-A or CY3-4040B or TRITC-A-Basic
DY-635-NHS	634	664	CY5-4040A
EBFP	380	440	DAPI-1160A or DAPI-5060B
ECFP	434	477	CFP-2432A
EGFP	489	508	GFP-3035B or GFP-A-Basic
Emerald	490	510	FITC-3540B or GFP-3035B
EYFP	513	527	YFP-2427A or YFP-A-Basic
Eosin	525	546	YFP-2427A or YFP-A-Basic
Ephedrine	260	390	TRP-A
Epinephrine	260	412	TRP-A
Estradiol	292	403	TRP-A
Ethidium Bromide	510	595	TRITC-A
Ethyl-p-dimethylaminobenzoate	310	330	TRP-A
FAM	492	518	FITC-3540B or FITC-A-Basic
Fast Blue	360	440	DAPI-1160A or DAPI-5060B
FDA	494	520	FITC-3540B
Feulgen (Pararosaniline)	570	625	TXRED-4040B
FIF (Formaldehyde Induced Fluorescence)	405	433	DAPI-1160A or DAPI-5060B
FITC (Fluorescein)	492	520	FITC-3540B or FITC-A-Basic
Fluo-3	506	526	YFP-2427A or YFP-A-Basic
Fluo-4	494	516	FITC-3540B or FITC-A-Basic
Fluoro-Emerald	495	524	FITC-3540B
Fluor-Ruby	555	582	CY3-4040B
FluorX	494	520	FITC-3540B
Fura-2	363, 336	512, 505	FURA2-B
Fura Red™ (high pH)	572	657	TXRED-4040B
Genacryl Brilliant Red B	520	590	TRITC-A
Genacryl Brilliant Yellow 10GF	430	485	CFP-2432A
Genacryl Yellow 5GF	430	475	CFP-2432A
GFP (EGFP)	489	508	GFP-3035B or GFP-A-Basic
Gloxalic Acid	405	460	DAPI-1160A or DAPI-5060B